NeuroMab™ Anti-NOTCH3 Antibody(NRP-0422-P1867)

Figure 1 is a bar graph summarizing the results of experiments using Octet to determine the specificity of antibody 4F11 binding to Fc-binding recombinant human Notch3 (rhNotch3). The results showed that the binding specificity of the 4F11 antibody to rhNotch3 was much higher than that to rhNotch1 or rhNotch2.

Figure 10 shows the summary results of the Notch3 reporter assay that humanized variants of 4F11 inhibit ligand Jag2-induced Notch3-dependent reporter expression.

Figure 2 is a bar graph illustrating the ability of antibody 4F11 to neutralize the binding of ligand Jag1 to human Notch3 protein.

Graph showing that antibody 4F11 and Notch3-specific controls, but not human immunoglobulin (hIgG), are able to block the binding of recombinant human Fc-bound Jag1 (Jag1-Fc) to rhNotch3 as detected by biolayer interferometry (BLI) of).

Figure 3 is a dose response curve summarizing results from a Notch3 reporter assay showing that antibody 4F11 inhibits Notch3-dependent reporter expression in the presence of the Fc-binding ligand Jag2 (Jag2-Fc).

Figure 4 is a bar graph summarizing the results of a Notch3 reporter assay showing that antibody 4F11, but not mouse IgG (mIgG), inhibits Notch3-dependent reporter gene expression induced by ligands hJagl, hJag2, hDLLl, and hDLL4.

Figure 5 is a graph illustrating that selected humanized 4F11 (i.e., Hu4F11-70, Hu4F11-72, Hu4F11-74, and Hu4F11-78) antibodies inhibit ligand-induced Notch3 ICD cleavage in MDA-MB-468 cells seeded on Jag2mFc diagram of the capabilities.

Figure 6 is a graph illustrating the ability of selected humanized 4F11 antibodies (ie, Hu4F11-70, Hu4F11-72, and Hu4F11-78) to inhibit Notch3 receptor activation in vivo following treatment of HCC2429 xenograft tumor-bearing mice.

Figure 7 is a bar graph illustrating the ability of antibody 4F11 to neutralize the binding of ligand Jag2 to human Notch3 protein. Ton

Figure 8 is a bar graph illustrating the ability of antibody 4F11 to neutralize the binding of ligand DLL1 to human Notch3 protein.

Figure 9 is a bar graph illustrating the ability of antibody 4F11 to neutralize the binding of ligand DLL4 to human Notch3 protein.