NeuroMab™ Anti-NOTCH3 Antibody(NRP-0422-P1847)

Product Overview

Description

The anti-Notch3 antibody binds to human Notch3 NRR region and useful for the treatment of cancer.

Immunogen

human Notch3 NRR region

Epitope

Fab

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Humanized

Applications

WB; ELISA; FC; Cyt; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease; Epilepsy; Pain

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

NOTCH3

Official Name

NOTCH3

Full Name

Notch 3

Alternative Names

Notch 3; Neurogenic Locus Notch Homolog Protein 3; Notch (Drosophila) Homolog 3; Notch Homolog 3 (Drosophila); Notch Homolog 3; CADASIL1;

Gene ID

4854(Human); 18131(Mouse); 56761(Rat)

Uniprot ID

Q9UM47(Human); Q61982(Mouse); Q9R172(Rat)

Product Pictures

Figure 1 shows Western blot analysis of Notch3 positive and negative cell lines using the D11B8 antibody.

Figure 3 shows Western blot analysis of the S2 cleavage assay using anti-Notch3 hu28 and hu75 treated HCC2429 and MDA-MB-468 cells. MW = molecular weight.

FuncS

Figure 4 shows that treatment of OVCAR3 ovarian cancer cells with anti-Notch3 hu28-vc0101 disrupts microtubules in mitotic cells (stained with anti-α-tubulin antibody), identified by phospho-histone H3 staining.

Figure 5 shows Western blot analysis of Notch3-ECD from xenografts harvested from 2-3 mice (M).

FuncS

Figure 6 shows the efficacy of anti-Notch3 hu28-vc0101 and hu75-vc0101 at a dose of 3 mg/kg compared to cisplatin at a dose of 5 mg/kg in a 37622A1 NSCLC patient-derived xenograft model.

Figure 2 Intracellular trafficking of anti-Notch3 antibody hu28-DyLight650 and co-localization with pHrodo™ red dextran in MDA-MB-468 breast cancer cells at 8 hours (arrows).