NeuroMab™ Anti-TREM2 Antibody(NRP-0422-P806)

Product Overview

Description

The antibody specifically binds to a human triggering receptor expressed on myeloid cells 2 (TREM2) and useful in preventing, reducing risk, or treating an individual in need.

Immunogen

Mouse Fc Fused Human TREM2 ECD

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Applications

FC; ELISA; Block; Inhib; In Vitro; In Vivo; Agonist

Relevant Diseases

Alzheimer's Disease

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

TREM2

Official Name

TREM2

Full Name

Triggering receptor expressed on myeloid cells 2

Alternative Names

TREM2; Triggering receptor expressed on myeloid cells 2

Gene ID

54209(Human)

Uniprot ID

Q9NZC2(Human)

Product Pictures

ELISA

Figure 1 Anti-TREM2 antibody binds to TREM2 on the cell surface.

Anti-TREM2 antibody surface binding dose response in hTREM2-HEK cells. Binding of anti-TREM2 antibodies was titrated to HEK cells expressing human TREM2. Antibody binding was detected with a secondary anti-mouse multiplexed antibody conjugated to APC. RS9.F6 and RS9.F10 demonstrate binding to human TREM2 cells with an EC50 of less than 2 nM.

FCM

Figure 2 Binding of anti-TREM2 antibodies to primary human macrophages.

Binding of anti-TREM2 antibodies to primary human monocyte-derived macrophages was screened by FACS. Antibody binding was detected with a secondary anti-mouse multiplexed antibody conjugated to APC. Gray line = secondary antibody alone. Black line = TREM2 antibody.

ELISA

Figure 3 Anti-TREM2 antibody activates P-Syk.

Determining the P-Syk Activating Dose Response of Anti-TREM2 Antibodies. Anti-TREM2 antibodies were dose titrated to HEK cells expressing human TREM2, and pSyk induction was calculated as a buffer control fold. EC50 values show the nM potency and fold induction of pSyk for each antibody.

Publications