iNeu™ Microglia AD TREM2 HZ, Disease Model
[CAT#: NCL-7P015]
Microglia Alzheimer's Disease differentiated from Human iPS cells, frozen
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- Microglia
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Microglia are immune cells that reside in the central nervous system (CNS) and are responsible for basic physiological and pathological processes. Microglia support neuronal homeostasis and promote the formation of neuronal networks through synaptic pruning. Surveillance Microglia can activate, adapt and respond to specific stimuli accordingly.
Microglial dysfunction is considered a key factor in neurodegenerative diseases, including Alzheimer's disease (AD). The limited availability and inconsistency of primary Human microglia limit research and treatment progress.
A rare variant of TREM2 (trigger receptor 2 expressed on bone marrow cells), an immune receptor expressed on microglia, is known to trigger bacterial phagocytosis and the release of reactive oxygen species, and is associated with AD patients The increased risk is related. Most TREM2 mutations found in AD risk variants are heterozygous mutations that affect TREM2 ligand binding or shedding of extracellular domains. The loss-of-function mutations of TREM2 and adaptor protein TYROBP are also related to the development of Nasu-Hakola disease, which is an inflammatory degenerative disease of the brain and bones, leading to premature dementia and death.
Engineering TREM2 model
In order to be able to study the functional consequences of TREM2 variants, the frameshift of TREM2 exon 2 is at amino acids near either allele (heterozygous; C60MfsTer45; iNeu Microglia TREM2 HZ) or two alleles (homozygous; P59AfsTer16 and P59AfsTer46) HO00) is designed in iPSCs. These iPSCs come from an apparently healthy and normal donor background, 01279.
Microglial dysfunction is considered a key factor in neurodegenerative diseases, including Alzheimer's disease (AD). The limited availability and inconsistency of primary Human microglia limit research and treatment progress.
A rare variant of TREM2 (trigger receptor 2 expressed on bone marrow cells), an immune receptor expressed on microglia, is known to trigger bacterial phagocytosis and the release of reactive oxygen species, and is associated with AD patients The increased risk is related. Most TREM2 mutations found in AD risk variants are heterozygous mutations that affect TREM2 ligand binding or shedding of extracellular domains. The loss-of-function mutations of TREM2 and adaptor protein TYROBP are also related to the development of Nasu-Hakola disease, which is an inflammatory degenerative disease of the brain and bones, leading to premature dementia and death.
Engineering TREM2 model
In order to be able to study the functional consequences of TREM2 variants, the frameshift of TREM2 exon 2 is at amino acids near either allele (heterozygous; C60MfsTer45; iNeu Microglia TREM2 HZ) or two alleles (homozygous; P59AfsTer16 and P59AfsTer46) HO00) is designed in iPSCs. These iPSCs come from an apparently healthy and normal donor background, 01279.
Cell Types
Microglia
Applications
Cell Assay
Species
Human
Properties
Size
1 Vial: ≥ 1 x 10^6 cells/vial
Tissue Source
Blood
Age
50-59
Mycoplasma Testing
The cell line has been screened using the Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination.
Shipping
Dry ice.
Storage
Liquid Nitrogen
Research Use Only
For research use only
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Target Details
Target
TREM2
Official Name
ADRA1A
Alternative Names
Alpha-1A adrenoceptor; alpha-1A adrenergic receptor
Donor Information
Donor Health
Alzheimer's Disease
Ethnicity
Caucasian
Additional Donor Information
Zygosity: Homozygous
Genotype: TREM2 (KO)
Genotype: TREM2 (KO)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
