iNeu™ Microglia AD TREM2 HO, Disease Model

Description

Microglia are immune cells that reside in the central nervous system (CNS) and are responsible for basic physiological and pathological processes. Microglia support neuronal homeostasis and promote the formation of neuronal networks through synaptic pruning. Surveillance Microglia can activate, adapt and respond to specific stimuli accordingly.

Microglial dysfunction is considered a key factor in neurodegenerative diseases, including Alzheimer's disease (AD). The limited availability and inconsistency of primary Human microglia limit research and treatment progress.

A rare variant of TREM2 (trigger receptor 2 expressed on bone marrow cells), an immune receptor expressed on microglia, is known to trigger bacterial phagocytosis and the release of reactive oxygen species, and is associated with AD patients The increased risk is related. Most TREM2 mutations found in AD risk variants are heterozygous mutations that affect TREM2 ligand binding or shedding of extracellular domains. The loss-of-function mutations of TREM2 and adaptor protein TYROBP are also related to the development of Nasu-Hakola disease, which is an inflammatory degenerative disease of the brain and bones, leading to premature dementia and death.

Engineering TREM2 model
In order to be able to study the functional consequences of TREM2 variants, the frameshift of TREM2 exon 2 is at amino acids near either allele (heterozygous; C60MfsTer45; iNeu Microglia TREM2 HZ) or two alleles (homozygous; P59AfsTer16 and P59AfsTer46) HO00) is designed in iPSCs. These iPSCs come from an apparently healthy and normal donor background, 01279.

Neurological Disease Models

AD-related Cells

Cell Types

Microglia

Relevant Diseases

Alzheimer's Disease

Species

Human

Size

1 Vial: ≥ 1 x 10^6 cells/vial

Tissue Source

Blood

Age

50-59

Mycoplasma Testing

The cell line has been screened using the Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination.

Shipping

Dry ice.

Storage

Liquid Nitrogen

Research Use Only

For research use only

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Target

TREM2

Official Name

ADRA1A

Alternative Names

Alpha-1A adrenoceptor; alpha-1A adrenergic receptor

Donor Health

Disease

Ethnicity

Caucasian

Additional Donor Information

Zygosity: Homozygous
Genotype: TREM2 (KO)