NeuroMab™ Mouse Anti-Human HDAC3 Monoclonal Antibody (CBP4342)

Product Overview

Description

HDAC3, also known as RPD3-2 and SMAP45, is a member of class I histone deacetylase (HDACs) family, which modulates the chromatin structure by removing acetyl groups (deacetylation) from the side chain of lysine residues on the N-terminal region of core histones. Generally, histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. It is known that HDAC3 deacetylase activity is stimulated by interacting with the N-CoR and SMRT co-repressor proteins and subsequently forms a functional complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1. HDAC-3 also represses gene expression by participating in the transcriptional repressor activity of BCL6, by deacetylating H3K27 on enhancer elements. HDAC3 is also thought to participate in transcriptional regulation through binding to YY1, a zinc-finger transcription factor. Recently, HDAC3 is known to be involved in the coordination of commensal-bacteria-dependent intestinal homeostasis when expressed in intestinal epithelial cells.

Immunogen

This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC3 protein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP4342

Applications

ChIP; IHC-P

Application Notes

ChIP (1:100-1:200); IHC-P (1:50)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 2309

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.