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Creative Biolabs
Product

Human CNTFR Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP137]

Human CNTFR knockout cell lysate

Species:
Human
Cell Types:
HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human CNTFR has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon6.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Signal Transduction

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

CNTFR

Official Name

CNTFR

Full Name

Ciliary Neurotrophic Factor Receptor

Alternative Names

Ciliary Neurotrophic Factor Receptor; CNTF Receptor Subunit Alpha; CNTFR-Alpha; Ciliary Neurotrophic Factor Receptor Subunit Alpha;

Gene ID

1271 (Human)

Uniprot ID

P26992 (Human); O88507 (Mouse); Q08406 (Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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