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Creative Biolabs
Product

Human GSS Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP316]

Human GSS knockout cell lysate

Species:
Human
Cell Types:
HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human GSS has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurogenesis

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

Glutathione Synthetase

Official Name

GSS

Full Name

Glutathione Synthetase

Alternative Names

Glutathione Synthetase; Glutathione Synthase; GSH Synthetase; EC 6.3.2.3; GSH-S; HEL-S-64p; HEL-S-88n; GSHS;

Gene ID

2937(Human); 14854(Mouse); 25458(Rat)

Uniprot ID

P48637(Human); P51855(Mouse); P46413(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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