Human GNB3 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP155]

Human GNB3 knockout cell lysate

Species:: Human

Cell Types:: HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human GNB3 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon8 and Insertion of the selection cassette in exon8.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

GNB3

Official Name

GNB3

Full Name

G Protein Subunit Beta 3

Alternative Names

G Protein Subunit Beta 3; Transducin Beta Chain 3; Guanine Nucleotide Binding Protein (G Protein); Beta Polypeptide 3; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Beta Subunit 3; Guanine Nucleotide-Binding Protein G(I)/G(S)/G(T) Subunit Beta-3; GTP-Binding Regulatory Protein Beta-3 Chain; Hypertension Associated Protein; G Protein; Beta-3 Subunit; CSNB1H;