Human GRK2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP418]

Human GRK2 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human GRK2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

GPCR

Pathways

GPCR Signaling

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

GRK2

Official Name

GRK2

Full Name

G Protein-Coupled Receptor Kinase 2

Alternative Names

G Protein-Coupled Receptor Kinase 2; EC 2.7.11.15; Beta-ARK-1; ADRBK1; BARK1; Adrenergic; Beta; Receptor Kinase 1; G-Protein Coupled Receptor Kinase 2;