Human KCNQ3 Knockout A549 Cell Lysate

[CAT#: NCL2008ZP179]

Human KCNQ3 knockout cell lysate

Species:: Human

Cell Types:: A549 Cell

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a A549 cell transfected lysate in which Human KCNQ3 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

A549 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon10 and 1 bp insertion in exon10 and 2 bp deletion in exon10.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Potassium Signaling

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12

Tissue Source

Lung

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

KCNQ3

Official Name

KCNQ3

Full Name

Potassium Voltage-Gated Channel Subfamily Q Member 3

Alternative Names

Potassium Voltage-Gated Channel Subfamily Q Member 3; Potassium Channel; Voltage Gated KQT-Like Subfamily Q; Member 3; Potassium Voltage-Gated Channel; KQT-Like Subfamily; Member 3; Voltage-Gated Potassium Channel Subunit Kv7.3; Potassium Channel Subunit Alpha KvLQT3; Potassium Channel; Voltage-Gated; Subfamily Q; Member 3;