- Host Species:
- Mouse
- Species Reactivity:
- Human; Mouse
- Applications:
- FC; ELISA; Block; Inhib; In Vitro; In Vivo; Agonist
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
The antibody specifically binds to a human triggering receptor expressed on myeloid cells 2 (TREM2) and useful in preventing, reducing risk, or treating an individual in need.
Immunogen
Mouse Fc Fused Human TREM2 ECD
Species Reactivity
Human; Mouse
Clonality
Monoclonal
Host Species
Mouse
Applications
FC; ELISA; Block; Inhib; In Vitro; In Vivo; Agonist
Relevant Diseases
Alzheimer's Disease
Product Properties
Formulation
PBS only
Preservatives
BSA Free
Concentration
1mg/mL
Purification
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purity
>95% by SDS PAGE or HPLC
Endotoxin Level
Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg
Low Endotoxin < 1 EU/mg
Shipping
Gel Packs
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Research Use Only
For research use only
Target
Product Pictures
ELISA
Figure 1 Anti-TREM2 antibody binds to TREM2 on the cell surface.
Anti-TREM2 antibody surface binding dose response in hTREM2-HEK cells. Binding of anti-TREM2 antibodies was titrated to HEK cells expressing human TREM2. Antibody binding was detected with a secondary anti-mouse multiplexed antibody conjugated to APC. RS9.F6 and RS9.F10 demonstrate binding to human TREM2 cells with an EC50 of less than 2 nM.
FCM
Figure 2 Binding of anti-TREM2 antibodies to primary human macrophages.
Binding of anti-TREM2 antibodies to primary human monocyte-derived macrophages was screened by FACS. Antibody binding was detected with a secondary anti-mouse multiplexed antibody conjugated to APC. Gray line = secondary antibody alone. Black line = TREM2 antibody.
ELISA
Figure 3 Anti-TREM2 antibody activates P-Syk.
Determining the P-Syk Activating Dose Response of Anti-TREM2 Antibodies. Anti-TREM2 antibodies were dose titrated to HEK cells expressing human TREM2, and pSyk induction was calculated as a buffer control fold. EC50 values show the nM potency and fold induction of pSyk for each antibody.
FuncS
Figure 4 Anti-TREM2 antibody induces human macrophage survival.
Human monocytes isolated from peripheral blood were incubated with 5 ng/mL M-CSF in the presence of titrated concentrations of plate-coated anti-TREM2 antibody or an isotype control. On day 6, cell viability was determined by the CellTiter Glo Viability Assay. Anti-TREM2 antibodies increase the survival of human macrophages cultured in limited or no M-CSF.
FuncS
Figure 5 Signaling pathway induced by anti-TREM2 antibody.
Human macrophages were stimulated with 30 nM antibody or control for 15 minutes. p-Y525/526-SYK of cell lysates was measured by Alpha-LISA. Anti-TREM2 antibody induces Syk phosphorylation, ERK phosphorylation, GSK3-β phosphorylation and AKT phosphorylation.
Publications
Publications (0)
Neural Cell Lines
Neural Antibodies
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentI have used the antibody for several in vitro assays, and the results have been consistently reliableThe antibody has high specificity and affinity, which has greatly improved the accuracy of my ELISA experiments. Additionally, its performance in flow cytometry is exceptional, providing clear and distinct populations.
ExcellentI highly recommend this product for anyone studying TREM2-related pathwaysWe've used it in both in vitro and in vivo studies, and the results have been outstanding. The antibody's blocking and inhibiting capabilities have provided us with crucial insights into TREM2's role in microglial activation.
ExcellentI've used the antibody for flow cytometry and ELISA assaysI am very pleased with its performance. The antibody shows excellent specificity, and the signal-to-noise ratio is impressive. It has helped us identify TREM2 expression in different cell populations with high precision.
ExcellentWe have had great success in our experimentsThe antibody's ability to inhibit TREM2 function in vitro has been particularly useful in our studies of neuroinflammation. It has also worked well in blocking assays, providing consistent and reproducible results. I highly recommend this antibody.
Q&As
Can the antibody be used in conjunction with other antibodies for double labeling experiments?
Yes, the antibody can be used for double labeling experiments. It is important to ensure that the secondary antibodies used are species-specific to avoid cross-reactivity. Proper optimization and validation of the double labeling protocol are crucial to obtain clear and specific results when using multiple antibodies in the same assay.
What is the concentration of the antibody provided in the vial?
The antibody is typically provided at a concentration of 1 mg/mL. This high concentration allows for multiple experiments and dilutions according to the requirements of different applications. Detailed concentration and storage instructions are provided with the product to ensure optimal use.
Is there any support available if I encounter issues?
Yes, comprehensive support is available for users of the antibody. Our technical support team is ready to assist with any issues or questions regarding the antibody’s use, optimization, and troubleshooting. You can contact our support team via email or phone, and we also provide detailed protocols and troubleshooting guides to help you achieve the best results in your experiments.
For Research Use Only. Not For Clinical Use.
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