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Creative Biolabs
Product

Human CNP Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP440]

Human CNP knockout cell lysate

Species:
Human
Cell Types:
HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human CNP has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 58 bp deletion in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neural Cell

Marker

Glial

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

Myelin CNPase

Official Name

CNP

Full Name

CNPase

Alternative Names

CNP; CNP1; 2';3'-cyclic nucleotide 3' phosphodiesterase; CNPase

Gene ID

1267 (Human); 12799 (Mouse); 25275 (Rat)

Uniprot ID

P09543 (Human); P16330 (Mouse); P13233 (Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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