Product

Human GRIN2D Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP431]

Human GRIN2D knockout cell lysate

Species:: Human

Cell Types:: HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human GRIN2D has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

GPCR

Pathways

GPCR Signaling

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

GRIN2D

Official Name

GRIN2D

Full Name

Glutamate Ionotropic Receptor NMDA Type Subunit 2D

Alternative Names

Glutamate Ionotropic Receptor NMDA Type Subunit 2D; Glutamate Receptor; Ionotropic; N-Methyl D-Aspartate 2D; Glutamate [NMDA] Receptor Subunit Epsilon-4; N-Methyl-D-Aspartate Receptor Subunit 2D; N-Methyl D-Aspartate Receptor Subtype 2D; NMDAR2D; GluN2D;

Publications