Adenosine A1 Receptor Assay Cell Line
[CAT#: NCL2110P310]
- Species:
- Human
- Cell Types:
- Other Cells
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
Cell line information: ADORA1 Maxensor Cell Line
Cell Types
Other Cells
Application Notes
About the Maxensor biosensor series:
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.
Research Areas
GPCR
Assay
Adenosine A1 Receptor Assay
Assay Description
Adenosine A1 receptor (ADORA1) is a member of the adenosine receptor group of GPCRs and uses adenosine as an endogenous ligand. The activation of the adenosine A1 receptor by the agonist leads to the binding of Gi1/2/3 or Go protein. The binding of Gi1/2/3 leads to the inhibition of adenylate cyclase, therefore, the concentration of cAMP is reduced. Creative Biolabs' Adenosine A1 receptor detection allows the use of Maxensor biosensors to measure ADORA1 receptor activation through Go activation.
Assay Characteristics
Readout: Gi/Go Activation
Agonist: NECA
EC50 Agonist: 2.19 x 106-8 M for Gi/Go Assay
Agonist: NECA
EC50 Agonist: 2.19 x 106-8 M for Gi/Go Assay
Species
Human
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
A1AR
Official Name
A1AR
Full Name
A1 receptor
Alternative Names
A1 receptor; Ri; RDC7; adenosine A1 receptor ; A1AR; A1R; A1-AR; AA1R; Ri
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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