Adenosine A1 Receptor Assay Cell Line

[CAT#: NCL2110P310]

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: ADORA1 Maxensor Cell Line

Cell Types

Other Cells

Application Notes

About the Maxensor biosensor series:
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.

Research Areas

GPCR

Assay

Adenosine A1 Receptor Assay

Assay Description

Adenosine A1 receptor (ADORA1) is a member of the adenosine receptor group of GPCRs and uses adenosine as an endogenous ligand. The activation of the adenosine A1 receptor by the agonist leads to the binding of Gi1/2/3 or Go protein. The binding of Gi1/2/3 leads to the inhibition of adenylate cyclase, therefore, the concentration of cAMP is reduced. Creative Biolabs' Adenosine A1 receptor detection allows the use of Maxensor biosensors to measure ADORA1 receptor activation through Go activation.

Assay Characteristics

Readout: Gi/Go Activation

Agonist: NECA

EC50 Agonist: 2.19 x 10⁶-8 M for Gi/Go Assay

Species

Human

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.