Human VANGL2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP333]

Human VANGL2 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human VANGL2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon4.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurogenesis

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

VANGL2

Official Name

VANGL2

Alternative Names

VANGL Planar Cell Polarity Protein 2; Vang-Like 2 (Van Gogh; Drosophila); Loop-Tail-Associated Protein; Loop-Tail Protein 1 Homolog; Van Gogh-Like Protein 2; Strabismus 1; STB1; Vang (Van Gogh; Drosophila)-Like 2; Vang; Van Gogh-Like 2 (Drosophila); Vang; Van Gogh-Like 2;