pLenti-CMV-GFP-EF1α-RFP-T2A-PURO Lentiviral Expression Vector (Reporter Plasmid)
- Applications:
- Virus Production
- Cell Types:
- Neural Lineage Cell
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Description
Lentiviral particles are produced from 293T cells by transient transfection of plasmids encoding viral particle components. For safety considerations regarding HIV-1 infectivity, recent lentiviral packaging systems have divided the viral components into 3 or 4 plasmids.
The pLenti-CMV-GFP-EF1α-RFP-T2A-PURO lentiviral reporter gene plasmid contains the CMV promoter before copGFP and carries the tdTomato reporter gene and the puromycin resistance gene driven by the EF1α promoter. This dual reporter gene vector was used as a positive control. Cells transduced by this vector should show green and red fluorescence.
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