pLenti-CMV-GFP-EF1α-RFP-T2A-PURO Lentiviral Expression Vector (Reporter Plasmid)

Description

Lentiviral vectors based on human immunodeficiency virus 1 (HIV-1) have become the most commonly used vectors for gene transfer research. The dominant feature of lentiviral vectors is the ability of gene transfer and integration into dividing and non-divi. The pseudotype envelope with vesicular stomatitis virus envelope G (VSV-G) protein expands the range of target cells. Lentiviral vectors have been shown to deliver genes to neurons, lymphocytes, and macrophages, while previous retroviral vectors cannot use this cell type. Lentiviral vectors have also been shown to effectively transduce brain, liver, muscle and retina in vivo without toxicity or immune response. Recently, lentivirus systems have been widely used to effectively integrate siRNA into various cell lines and primary cells in vitro and in vivo.

Lentiviral particles are produced from 293T cells by transient transfection of plasmids encoding viral particle components. For safety considerations regarding HIV-1 infectivity, recent lentiviral packaging systems have divided the viral components into 3 or 4 plasmids.

The pLenti-CMV-GFP-EF1α-RFP-T2A-PURO lentiviral reporter gene plasmid contains the CMV promoter before copGFP and carries the tdTomato reporter gene and the puromycin resistance gene driven by the EF1α promoter. This dual reporter gene vector was used as a positive control. Cells transduced by this vector should show green and red fluorescence.

Cell Types

Neural Lineage Cell

Species Reactivity

Human; Mouse; Rat

Applications

Virus Production

Research Areas

Stem Cell Research

Shipping

Room Temperature

Storage

Store at -20°C

Handling Advices

For all materials containing BSL-2 organisms, follow the recommended NIH guidelines. The NeuroShop Lentiviral Expression System is designed to minimize the chance of producing lentivirus with replication capabilities, but precautions should still be taken to avoid direct contact with viral supernatant.

Quality Control

This plasmid is sequence verified.

Shelf Life

This product is stable for 6 months when stored as directed.

Research Use Only

For research use only, not for diagnostic or therapeutic use.