Human CELF1 Knockout HEK293T Cell Lysate
[CAT#: NCL2008ZP512]
Human CELF1 knockout cell lysate
- Species:
- Human
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a HEK293T cell transfected lysate in which Human CELF1 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
Other Cells
Cell Location
Epithelial
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon 2 and 5 bp deletion in exon 2.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Research Areas
Neurodegeneration
Species
Human
Properties
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target Details
Target
CUG-BP1
Official Name
CELF1
Full Name
CUG triplet repeat, RNA binding protein 1,
Alternative Names
CELF1; BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; NAB50; NAPOR; hNab50; CUGBP; Elav-like family member 1; CUGBP Elav-like family member 1
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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