NeuroMab™ Anti-CD284 Antibody(NRP-0422-P744)

Figure 1 is a graph depicting the binding of a murine monoclonal antibody (referred to herein as "15C1") to the TLR4/MD-2 complex.

Cells were incubated with indicated concentrations of mu18H10, HTA 125 (commercially available anti-human TLR4 non-blocking monoclonal antibody) or antibody control followed by incubation with LPS (15 ng/ml). IL-8 levels were assessed 16 hours after LPS treatment.

Figure 2 is a graph depicting inhibition of lipopolysaccharide (LPS)-induced IL-8 production in TLR4/MD-2 transfected HEK 293 cells by monoclonal antibody mu15C1.

Cells were incubated with indicated concentrations of mu18H10, HTA 125 (commercially available anti-human TLR4 non-blocking monoclonal antibody) or antibody control followed by incubation with LPS (15 ng/ml). IL-8 levels were assessed 16 hours after LPS treatment.

Figure 3 depicts the inhibition of LPS-induced IL-6 production in human whole blood by monoclonal antibody mu15C1.

Cells were incubated with indicated concentrations of mu18H10, HTA 125 (commercially available anti-human TLR4 non-blocking monoclonal antibody) or antibody control followed by incubation with LPS (15 ng/ml). IL-8 levels were assessed 16 hours after LPS treatment.

Figure 4 is a series of graphs depicting the specificity of mu15C1 monoclonal antibody for TLR4.

The specificity of the mu15C1 antibody was shown by flow cytometric analysis of HEK 293 cells transiently transfected with either mock vector, namely empty vector (panel A), human TLR4 (panel B), human TLR4 and human MD-2 (panel C ), rabbit TLR4 and rabbit MD-2 (Figure D).

Figure 5 is a graph depicting the ability of the VH and VL nucleotide sequences of mu15C1 expressed as a chimeric MAb ("chimeric 15C1") to specifically bind human TLR4/MD-2 complexes on the surface of transfected CHO cells.

The specificity of the mu15C1 antibody was shown by flow cytometric analysis of HEK 293 cells transiently transfected with either mock vector, namely empty vector (panel A), human TLR4 (panel B), human TLR4 and human MD-2 (panel C ), rabbit TLR4 and rabbit MD-2 (Figure D).

Figure 6 is a graph depicting inhibition of lipopolysaccharide (LPS)-induced IL-8 production by chimeric 15C1 MAb in TLR4/MD-2 transfected HEK 293 cells.

The specificity of the mu15C1 antibody was shown by flow cytometric analysis of HEK 293 cells transiently transfected with either mock vector, namely empty vector (panel A), human TLR4 (panel B), human TLR4 and human MD-2 (panel C ), rabbit TLR4 and rabbit MD-2 (Figure D).