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Creative Biolabs
Product

NeuroMab™ Anti-Plexin A1 Antibody, Clone PXB361b

[CAT#: NRP-0422-P590]

A functional antibody raised against Mouse PlexinA1.

Host Species:
Rabbit
Species Reactivity:
Mouse
Applications:
ELISA; In Vitro; Agonist

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Product Overview

Description

The antibody exhibits binding affinities only for mouse PlexinA1. This a novel anti-Plexin-A1 agonist antibody that promotes dendritic cell contraction.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rabbit

Clone Number

PXB361b

Applications

ELISA; In Vitro; Agonist
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

Plexin A1

Official Name

PLXNA1

Full Name

Plexin-A1

Alternative Names

PLXNA1; NOV; NOVP; PLEXIN-A1; PLXN1; Plexin A1

Gene ID

5361(Human); 18844(Mouse); NCBI: NP_115618(Rat)

Uniprot ID

Q9UIW2(Human); P70206(Mouse)
Product Pictures
ELISA

Figure 1 is a graph showing the binding activity of an anti-mouse PlexinA1 antibody to mouse PlexinA1.

The X-axis represents the antibody concentration, and the Y-axis represents the absorbance at a wavelength of 405 nm. Solid lines indicate binding to Ba/F3 cell line expressing high levels of mouse PlexinA1, dashed lines indicate binding to Ba/F3 cells.

Antangonist

Figure 2 is a graph showing the dose-dependent antagonistic activity of a control antibody or an anti-PlexinA1 antibody (rabbit IgG) at a concentration of 0 to 100 µg/mL on the contraction of semaphorin 3A-dependent mouse bone marrow-derived dendritic cells.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

ELISA

Figure 3 illustrates a graph showing the β-β measured by immobilizing the mouse chimeric antibodies hPANL#240-mFc, 359B-2-2-3-6-mFc, PXB693-mFc, PXB727-mFc, and PXB361b-mFc onto a Protein G biosensor. Plot of the binding reaction and applying human PlexinA1 and mouse PlexinA1 on it.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

IP

Figure 4 illustrates a graph showing the binding affinity of the antagonist antibody mouse chimera PXB361b to mouse PlexinAl, mouse PleixinA2, and chimeric proteins of mouse PlexinAl and PlexinA2. 1: mouse PlexinA1 sema domain, 2: mouse PlexinA1/A2 sema domain chimera, 3: mouse PlexinA2 sema domain, Control Ab: control antibody, PXB361b: mouse chimeric antibody PXB361b-mFc.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

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