NeuroMab™ Mouse Anti-CD163 Monoclonal Antibody (CBP2567)

Product Overview

Description

The mouse anti-rat CD163 recognizes rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule, also known as rat CD163, is a member of the cysteine-rich (SRCR) family of group B scavenger receptors and is an erythroblast adhesion receptor (Fabriek et al., 2007). Mouse anti-rat CD163 clone ED2 has been shown to detect about 50% of peritoneal macrophages, a part of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al., 1985; Beelen et al., 1987). In newly isolated bone marrow, the expression of CD163 is restricted to mature macrophages (Barbe et al., 1990).

Immunogen

Rat spleen cell homogenate.

Species Reactivity

Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2567

Applications

Application Notes

FC: 1/10

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1868

Product Properties

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide, 1% BSA

Preservatives

YES

Concentration

0.05 mg/ml

Purification

Protein A

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD163

Official Name

CD163

Full Name

CD163 Molecule

Alternative Names

CD163 Molecule; Hemoglobin Scavenger Receptor; CD163 Antigen; M130; Scavenger Receptor Cysteine-Rich Type 1 Protein M130; Macrophage-Associated Antigen; SCARI1; MM130;

Gene ID

9332(Human); 93671(Mouse); 312701(Rat)

Uniprot ID

Q86VB7(Human); Q2VLH6(Mouse)

References

1. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation.
2. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
3. Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
5. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.

Publications