Human CMEC/D3 Cell Line, Blood-Brain Barrier Model

The hCMEC/D3 BBB cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood-brain barrier (BBB) function.

Species:: Human

Applications:: Cell Culture

Cell Types:: BBB-related Cells

Cell Subtypes:: Brain Endothelial Cells

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Product Overview

Description

The human temporal lobe microvessels that were obtained from tissue that was removed during surgery to treat epilepsy gave rise to the hCMEC/D3 cell line. The cerebral endothelial cells (CECs) in the primary isolate were enriched. The catalytic subunit of human telomerase (hTERT) and the SV40 large T antigen were sequentially used to immortalise cells during the first passage. CEC were then selectively isolated by limited dilution cloning, and clones were thoroughly examined for brain endothelial phenotype. This brain microvascular endothelial cell line is one such readily cultivable model of the human BBB that allows for cellular and molecular research on disease and drug transport pathways relevant to the central nervous system (CNS).

Cell Types

BBB-related Cells

Cell Subtypes

Brain Endothelial Cells

Applications

Cell Culture

Application Notes

The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.

Species

Human

Properties

Size

>1x10^6 Cells

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

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Scientific Resources

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

This cell line is a great resource for BBB studies

The CMEC/D3 cell line has been a reliable model for studying the BBB in vitro. We used it to investigate drug permeability, and the results were consistent with in vivo data. The cells were easy to culture and maintained their characteristic morphology throughout the experiments. Highly recommend it for BBB-related studies.

Excellent

Ease of culturing and reproducibility of the results

These cells offer a robust and reproducible model for the human blood-brain barrier. We utilized the CMEC/D3 cell line for co-culture experiments with astrocytes, and the barrier properties were well-maintained. The tight junction integrity was particularly impressive, as confirmed by TEER measurements.

Excellent

The cells are robust and easy to work with

The CMEC/D3 cells are an excellent tool for modeling the BBB in drug delivery studies. They form a strong, consistent barrier that mimics the in vivo environment well. My team has been using them to test nanoparticle delivery systems, and the data we've gathered has been invaluable.

Excellent

These cells are an essential tool for our lab

Our lab has been using the CMEC/D3 cell line to study neuroinflammation, and it has performed exceptionally well. The cells responded to cytokine treatments as expected, making it a reliable model for understanding BBB dynamics under inflammatory conditions.

Excellent

We've observed excellent barrier properties

The CMEC/D3 cells provided us with a high-quality in vitro model for BBB research. We used them to study the effects of hypoxia on endothelial permeability and found the results to be highly reproducible. The cells are easy to handle, and their growth characteristics are very stable.

Q&As

Can I co-culture the human CMEC/D3 cells with astrocytes or pericytes to create a more complex BBB model?

Yes, the human CMEC/D3 cell line can be co-cultured with astrocytes, pericytes, or other glial cells to create a more physiologically relevant BBB model. Co-culturing can enhance the expression of tight junction proteins and improve barrier properties, as the interactions between endothelial cells and supporting cells better mimic the in vivo environment. This approach can provide more comprehensive insights into BBB function and pathology.

What types of assays can I perform with the CMEC/D3 cell line to study BBB function?

The CMEC/D3 cell line can be used in a variety of assays to study BBB function. Common assays include permeability assays using small molecules or macromolecules to assess the selective transport across the BBB, TEER measurements to evaluate barrier integrity, and cytokine treatment studies to understand the impact of inflammation on BBB disruption. Additionally, the cells can be used in co-culture systems to study cell-cell interactions within the BBB.

What precautions should I take when handling the CMEC/D3 cells to maintain their BBB characteristics?

To maintain the BBB characteristics of the CMEC/D3 cells, it is essential to follow specific culture practices. Avoid over-confluency by passaging the cells before they reach 90% confluence, as overcrowded cells can lose their tight junction integrity. Regularly monitor TEER values and tight junction protein expression to ensure the cells maintain their barrier properties. Additionally, use high-quality, low-passage cells to minimize variability in experimental outcomes.