Immortalized Human Cerebral Microvascular Endothelial Cells

Model of human blood-brain barrier

Species:: Human

Cell Types:: BBB-related Cells

Cell Subtypes:: Brain Endothelial Cells

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Product Overview

Description

Immortalized human cerebral microvascular endothelial cells (HCMECs) are a cell line that can be used to model the blood-brain barrier (BBB) in vitro. They are a less expensive alternative to primary HBMECs. HCMECs are useful for studying drug uptake and active transport studies.

Cell Types

BBB-related Cells

Cell Subtypes

Brain Endothelial Cells

Species

Human

Properties

Size

1M cells/vial

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Shipping

Dry Ice

Storage

Liquid nitrogen

Handling Advice

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Research Use Only

For research use only

Warnings

Negative for mycoplasma

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

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Scientific Resources

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

This cell line is a great resource for BBB studies

The CMEC/D3 cell line has been a reliable model for studying the BBB in vitro. We used it to investigate drug permeability, and the results were consistent with in vivo data. The cells were easy to culture and maintained their characteristic morphology throughout the experiments. Highly recommend it for BBB-related studies.

Excellent

Ease of culturing and reproducibility of the results

These cells offer a robust and reproducible model for the human blood-brain barrier. We utilized the CMEC/D3 cell line for co-culture experiments with astrocytes, and the barrier properties were well-maintained. The tight junction integrity was particularly impressive, as confirmed by TEER measurements.

Excellent

The cells are robust and easy to work with

The CMEC/D3 cells are an excellent tool for modeling the BBB in drug delivery studies. They form a strong, consistent barrier that mimics the in vivo environment well. My team has been using them to test nanoparticle delivery systems, and the data we've gathered has been invaluable.

Excellent

These cells are an essential tool for our lab

Our lab has been using the CMEC/D3 cell line to study neuroinflammation, and it has performed exceptionally well. The cells responded to cytokine treatments as expected, making it a reliable model for understanding BBB dynamics under inflammatory conditions.

Excellent

We've observed excellent barrier properties

The CMEC/D3 cells provided us with a high-quality in vitro model for BBB research. We used them to study the effects of hypoxia on endothelial permeability and found the results to be highly reproducible. The cells are easy to handle, and their growth characteristics are very stable.

Q&As

Can I co-culture the human CMEC/D3 cells with astrocytes or pericytes to create a more complex BBB model?

Yes, the human CMEC/D3 cell line can be co-cultured with astrocytes, pericytes, or other glial cells to create a more physiologically relevant BBB model. Co-culturing can enhance the expression of tight junction proteins and improve barrier properties, as the interactions between endothelial cells and supporting cells better mimic the in vivo environment. This approach can provide more comprehensive insights into BBB function and pathology.

What types of assays can I perform with the CMEC/D3 cell line to study BBB function?

The CMEC/D3 cell line can be used in a variety of assays to study BBB function. Common assays include permeability assays using small molecules or macromolecules to assess the selective transport across the BBB, TEER measurements to evaluate barrier integrity, and cytokine treatment studies to understand the impact of inflammation on BBB disruption. Additionally, the cells can be used in co-culture systems to study cell-cell interactions within the BBB.

What precautions should I take when handling the CMEC/D3 cells to maintain their BBB characteristics?

To maintain the BBB characteristics of the CMEC/D3 cells, it is essential to follow specific culture practices. Avoid over-confluency by passaging the cells before they reach 90% confluence, as overcrowded cells can lose their tight junction integrity. Regularly monitor TEER values and tight junction protein expression to ensure the cells maintain their barrier properties. Additionally, use high-quality, low-passage cells to minimize variability in experimental outcomes.