Tel:
Fax:
Email:
Creative Biolabs
Product

Human UBE2K Knockout HCT116 Cell Lysate

[CAT#: NCL2008ZP559]

Human UBE2K knockout cell lysate

Species:
Human
Cell Types:
HCT116 Cell

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry
Product Overview

Description

This is a HCT116 cell transfected lysate in which Human UBE2K has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HCT116 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Relevant Diseases

Huntington's Disease

Research Areas

Neurodegeneration

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

UBE2K

Official Name

UBE2K

Alternative Names

UBE2K; E2-25K; HIP2; HYPG; LIG; UBC1; ubiquitin conjugating enzyme E2 K

Gene ID

3093(Human); 53323(Mouse); 289623(Rat)

Uniprot ID

P61086(Human); P61087(Mouse)
Publications

Publications (0)

gift-card

Related Products
For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Send inquiry