NeuroMab™ Mouse Anti-CD14 Monoclonal Antibody (CBP2522)

Product Overview

Description

Mouse anti-human CD14 antibody, recognizes human CD14 cell surface antigen. CD14 is a glycoprotein of approximately 55 kDa, containing multiple leucine-rich repeat sequences. It is anchored to the cell membrane through glycosylphosphatidylinositol (GPI) linkages (Simmons et al., 1989), and there is also a soluble form of CD14 (Bazil et al., 1986). CD14 is strongly expressed on the surface of monocytes and macrophages, but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 plays a role as a pattern recognition receptor in innate immunity to various ligands, especially LPS (endotoxin) of Gram-negative bacteria (Pugin et al., 1994; Dziarski et al., 1998).

Species Reactivity

Human; Dog; Goat; Cat; Rabbit; Mink; Bovine; Porcine; Sheep; Cynomolgus monkey; Llama

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2522

Applications

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1846

Product Properties

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide, 1% BSA

Preservatives

YES

Concentration

0.05 mg/ml

Purification

Protein A

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD14

Official Name

CD14

Full Name

CD14 Molecule

Alternative Names

CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein

Gene ID

929(Human); 12475(Mouse); 60350(Rat)

Uniprot ID

P08571(Human); P10810(Mouse); Q63691(Rat)

References

1. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
2. Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
3. Xiong, W. et al. (2010) Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial.
4. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.

Publications