NeuroMab™ Mouse Anti-CD14 Monoclonal Antibody (CBP1940)

Product Overview

Description

Mouse anti-bovine CD14 clone CC-G33 is a monoclonal antibody that recognizes bovine CD14. Bovine CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen. It belongs to the family of lipopolysaccharide receptors and is found in microglia. It is also weakly expressed on cells and Langerhans cells.
CD14 acts as a receptor for effective bacterial endotoxin lipopolysaccharide (LPS) and is promoted by LPS binding protein (LBP). The binding of LPS to CD14 leads to cell activation, cytokine release and inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.

Immunogen

Partially purified polypeptide isolated from the surface membrane of bovine leukocytes.

Species Reactivity

Bovine; Sheep; Human; Water Buffalo

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1940

Applications

Relevant Diseases

Lou Gehring's Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1575

Product Properties

Form

Liquid

Formulation

PBS; 0.09% Sodium Azide
1% Bovine Serum Albumin

Preservatives

Yes

Concentration

0.1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD14

Official Name

CD14

Full Name

CD14 Molecule

Alternative Names

CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein

Gene ID

929(Human); 12475(Mouse); 60350(Rat)

Uniprot ID

P08571(Human); P10810(Mouse); Q63691(Rat)

References

1. Berthon, P. & Hopkins, J. (1996) Ruminant cluster CD14.
2. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
3. Harris, J. et al. (2003) Expression of caveolin by bovine lymphocytes and antigen-presenting cells.
4. Yamakawa, Y. et al. (2008) Identification and functional characterization of a bovine orthologue to DC-SIGN.
5. Herath, S. et al. (2006) Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus.
6. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.

Publications