NeuroMab™ Rat Anti-CD80 Monoclonal Antibody (CBP2554)

Product Overview

Description

The rat anti-Mouse CD80 antibody, recognizes mouse CD80 (B7-1), a cell surface glycoprotein of about 60 kDa, which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and the expression of LPS and IL-4 on B lymphocytes may increase significantly. CD80 has been identified as a ligand for CD28 and cytotoxic T lymphocyte antigen 4 (CTLA-4), which are two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors with basic but opposite functions in T cell stimulation. The interaction of CD80 and CD28 stimulates and maintains T cell responses, while the interaction of CD80 and CTLA4 is reported to inhibit T cell responses.

Immunogen

BCL1 cells express CD80.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a

Clone Number

CBP2554

Applications

Application Notes

FC: 1/5

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1862

Product Properties

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide, 1% BSA

Preservatives

YES

Purification

Protein G

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD80

Official Name

CD80

Full Name

CD80 Molecule

Alternative Names

CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1;

Gene ID

941(Human); 12519(Mouse)

Uniprot ID

P33681(Human); Q00609(Mouse)

References

1. Jin LP et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
2. Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
3. Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.

Publications