NeuroMab™ Anti-Human B7-1/CD80 Antibody, Clone 37711 (CBP9076)

Product Overview

Description

Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80 Val35-Asn242 (predicted)

Construction

Reconstitute at 0.5 mg/mL in sterile PBS.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Clone Number

CBP9076

Applications

FC; IHC; Dual RNAscope ISH-IHC; ELISA

Application Notes

Flow Cytometry: 0.25 µg/106 cells
Immunohistochemistry: 8-25 µg/mL
Dual RNAscope ISH-IHC: 5-25 µg/mL
ELISA: 2-8 µg/mL

Marker

B7-1/CD80

Conjugation

Unconjugated

Product Properties

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purification

Protein A or G purified from hybridoma culture supernatant.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Research Use Only

For research use only

Target

CD80

Official Name

CD80

Full Name

CD80 molecule

Alternative Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80