Mouse Anti-Human CD25 Monoclonal Antibody (CBP3866)

[CAT#: NAB2012417LS]

Anti-CD25 Monoclonal Antibody [Alexa Fluor 647 ]

Host Species:: Mouse

Species Reactivity:: Human

Applications:: FC

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Product Overview

Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.

Immunogen

Human PHA-induced lymphocyte cells

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP3866

Applications

Application Notes

FCM (5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Alexa Fluor 594; Alexa Fluor 488; Alexa Fluor 498; Alexa Fluor 647; Alexa Fluor 700; APC; APC/Cyanine 7; APC/Fire 750; Biotin; Brilliant Violet 421; Brilliant Violet 510; Brilliant Violet 570; Brilliant Violet 570; Brilliant Violet 605; Brilliant Violet 650; Brilliant Violet 711; Brilliant Violet 750; Brilliant Violet 785; FITC; HRP; PE; Pacific Blue; PE/Cyanine 5; PE/Cyanine 7; PerCP; Spark YG 570

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD25

Official Name

Il2RA

Full Name

interleukin 2 receptor, alpha chain

Alternative Names

IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Gene ID

3559(Human);16184(Mouse)

Uniprot ID

P01589(Human);P01590(Mouse)

References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Publications