Sensory Neuron Maturation Plus Medium Kit

Description

The kit contains 1 bottle of 250 mL Sensory Neuron Maintenance Medium and 4 vials of the Maturation Plus.

Maturation Plus is a fully defined medium supplement for enhancing the existing Sensory Neuron Maintenance Medium (Cat. NCC-2101-ZP15). It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.

iPSC-derived sensory neurons treated with the Maximizer express key markers, such as TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8. Kinetic states of e.g. TRPv1 are in a fast response inward rectifying state, to agonists such as Capsaicin. Neuronal responses to capsaicin occur at 1μM for 90% of the sensory neuron population on day 22 of culture.

The Maturation Plus supplement makes the Human iPSC-derived sensory neuronal culture a more physiological relevant model.

Cell Types

Neuron

Applications

Cell Culture

Application Notes

The medium requires supplementation with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)

Components

1 x 250mL bottle of Sensory Neuron Maintenance Medium (Cat. NCC-2101-ZP15)
1 x 1ml vial of MATURATION MAXIMISER supplement

Shelf Life

Dry ice

Research Use Only

-80°C