iPS Cell Line β-Actin GFP (Human)

[CAT#: NCL200505ZP]

iPS cell line derived from OSKM retrovirus. Exogenous gene silencing and concomitant expression of corresponding endogenous genes have been evaluated.

Species:: Human

Cell Types:: iPSC

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Product Overview

Description

By using ICC and/or PCR techniques, it has been demonstrated that iPS cell lines can express typical pluripotency markers (such as Tra1-60, Oct3/4, and nanog). Additionally, it has been demonstrated that this cell line is karyotype-normal and that it still retains the capacity to spontaneously differentiate into all three germ layers.

Cell Types

iPSC

Species

Human

Properties

Size

Each vial contains ≥ 1X10e6 viable cells.

Form

Frozen Cryovials/Live Culture

Tissue Source

Human (Caucasian female)

Age

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Cells are negative for mycoplasma contamination.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.

Donor Information