Creative Biolabs

iPS Cell Line β-Actin GFP (Human)

[CAT#: NCL200505ZP]

iPS cell line derived from OSKM retrovirus. Exogenous gene silencing and concomitant expression of corresponding endogenous genes have been evaluated.

Species:
Human
Applications:
Cell Assay
Cell Types:
Neural Stem Cell; Neural Progenitor Cell; iPSC

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Description
Features and benefits
It has been shown that iPS cell lines can express normal pluripotency markers (ie Tra1-60, Oct3 / 4, nanog) by ICC and / or PCR methods. It has also been shown that this cell line retains the ability to differentiate into all three germ layers by spontaneous embryoid body differentiation, and has been proven to be karyotype normal.
Cell Types
Neural Stem Cell; Neural Progenitor Cell; iPSC
Applications
Cell Assay
Relevant Diseases
Neurological Disorders
Species
Human
Size
Each vial contains ≥ 1X10e6 viable cells.
Form
Frozen Cryovials/Live Culture
Cell State
Live cells are in mid log-phase growth.
Tissue Source
Human (Caucasian female)
Age
24
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Cells are negative for mycoplasma contamination.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.
Gender
Female
Donor Age
24
Ethnicity
Caucasian

For Research Use Only. Not For Clinical Use.
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