Peripheral Blood Mononuclear Cell (PBMC)-Derived iPS Cell Line, Episomal (Human)

[CAT#: NCL200545ZP]

A footprint-free human iPS (induced pluripotent stem) cell line was derived from human peripheral blood mononuclear cells (PBMCs) by ectopic expression of OCT4, SOX2, KLF4, and L-MYC genes using episomal plasmids.

Species:: Human

Cell Types:: iPSC

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Product Overview

Description

The cells were derived using morphological selection criteria. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is the same as human ES cells. These cells also expressed pluripotency markers TRA-1-60, SSEA-3 and Oct4, and showed strong endogenous alkaline phosphatase activity.

Cell Types

iPSC

Marker

TRA-1-60/ Oct4

Species

Human

Properties

Size

2x10e5 cells/vial

Form

Frozen Cryovials/Live Culture

Tissue Source

Human peripheral blood mononuclear cells (PBMCs)

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Cells are negative for mycoplasma contamination.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Human iPS cells were cultured in mTeSR1 medium without feeder layers. After recovery from cryopreservation, each batch of human iPS cells was tested for growth and viability. In addition, each batch was tested for TRA-1-60 and Oct4 expression and alkaline phosphatase activity.

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