NeuroMab™ Anti-CXCL13 BBB Shuttle Antibody(NRZP-1022-ZP3436)

Figure 1. Specific ELISA results of binding of MAb 5261, MAb 5080, MAb 1476 and human isotype control to recombinant human (A), cynomolgus monkey (B) and mouse (C) CXCL13.

Each data point represents the mean of triplicate measurements. EC50 values were calculated from a four-parameter sigmoidal curve fit (curves shown; R2 of the curve yielding EC50 values was 0.99). NB=No binding.

Figure 2. Epitope competition ELISA results of MAb 5261, MAb 5080 and 3D2 binding to native human (A) and native mouse (B) CXCL13.

Each data point represents an average of duplicate measurements from one of at least three independent experiments. Curves were fitted using four-parameter sigmoidal curve fit (curves are shown on the graph; R2=0.99).

Figure 3. Percent inhibition of migration of human pre-B-697-hCXCR5 (A) and human tonsillar cells (B) by MAb 5261.

Data represent an average of triplicate measurements +/−SEM from one of at least three experiments. Curves were fitted using four-parameter sigmoidal curve fit (curves are shown on the graph; R2=0.98-0.99).

Figure 4. Percent inhibition of SJL/J splenocyte migration by MAb 5261.

Data from representative experiments are shown as mean of duplicate measurements +/−SD.

Figure 5. Percent inhibition of human CXCL13-mediated internalization of human CXCR5 receptor by MAb 5261 and isotype control.

Data are average of triplicate measurements from one of at least three independent experiments. Curve was fitted using four-parameter sigmoidal curve fit (curves are shown on the graph; R2=0.99).

Figure 6. Epitope competition ELISA results for MAb 5378, MAb 5261 and MAb 470.

Figure 7. Percent inhibition of migration of human pre-B-697-hCXCR5 (A), human tonsil cells (B) and C57Black6 mouse splenocytes (C) by MAb 5261 or MAb 5378 and MAb 5378 or 3D2.

Data represent an average of triplicate measurements +/−SEM from one of at least three experiments. Curves were fitted using four-parameter sigmoidal curve fit (curves are shown on the graph; R2=0.99).

Figure 8. Percent inhibition of human CXCL13-mediated internalization of the human CXCR5 receptor by MAb 5378, MAb 5261, 3D2, mouse isotype control, or human isotype control.

Data points for 5261 and 5378 represent average of measurements from two independent experiments. Data points for 3D2 and Isotype Controls represent average of triplicate measurements from a single experiment. Curve was fitted using four-parameter sigmoidal curve fit (curves are shown on the graph; R2=0.99). NE=no effect.