NeuroMab™ Anti- mGluR1/5 (group I) glutamate receptor BBB Shuttle Antibody, Clone N75/33

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-mGluR5 BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (also known as metabotropic glutamate receptor 5, accession number P31424)
Mouse: 96% identity (368/388 amino acids identical)
Human: 87% identity (339/388 amino acids identical)
<35% identity with mGluR1

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Isotype

IgG1

Clone Number

N75/33

Applications

WB; IHC; IP; ICC

Application Notes

Recognizes mGluR1 and mGluR5

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

mGluR5

Official Name

GRM5

Full Name

mGluR1/5 (Group I) glutamate receptor

Alternative Names

GRM5; GPRC1E; MGLUR5; PPP1R86; mGlu5; glutamate metabotropic receptor 5

Uniprot ID

P31424

Product Pictures

Fig. 1 Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].

Fig. 2 Adult rat brain membrane immunoblot.

IHC

Fig. 3 Adult rat hippocampus immunohistochemistry.

Fig. 4 Electron micrograph shows N75/33 immunolabelling in the stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunogold particles were observed at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts (Den) of pyramidal cells establishing synaptic contacts with excitatory axon terminals (b). 80.000 X.

Publications