NeuroMab™ Anti-Nav1.7 BBB Shuttle Antibody(NRZP-1022-ZP2489)

Fig.1 shows ELISA responses of 1E16 mAb and 115 mAb using intact Nav1.7 DII VSD. Data are shown as means S.E.M (n=3).

1080 monoclonal antibody produces a ˜26% inhibition of Nav1.7 currents at 25 μg/ml.

Fig.2 shows the voltage-sensor-targeting 1E16 inhibits human Nav1.7 in HEK293 cells.

Representative current traces from HEK293 cells expressing hNav1.7 in the absence or presence of 1 iuM 115 (A) and 100 nM 1E16 (C). Current-voltage relationships in the absence (o) or presence (D) of 1 iuM 115 (B) and 100 nM 1E16 (D) were generated using 30 ms voltage steps between -80 and +60 mV with 10 mV increments from a holding potential of -120 mV.

Fig.3 shows 1E16 inhibits Nav1.7 in a subtype-specific and state-dependent manner in HEK293 cells.

State (use)-dependent inhibition of human Nav1.7 by 1E16.

Fig.4 shows the effects of the 1E16 mAb on Nav1.7 resulted from specific interactions between the tip (loop) of the voltage-sensor paddle and 1E16.

Representative traces and current¨voltage relationship from HEK293 cells expressing human Nav1.7 channels (A), in the presence of 1E16 (100 nM) and the peptide (1 ilM) (B), and after washout in the presence of 1E16 (100 nM) only (C).

Fig.5 shows the effect of the mAb on persistent sodium currents (INaPs) in small-sized neurons of whole mount dorsal root ganglions (DRGs) from naïve mice and mice with nerve injury (CCI.).

Representative traces and current¨voltage relationship from HEK293 cells expressing human Nav1.7 channels (A), in the presence of 1E16 (100 nM) and the peptide (1 ilM) (B), and after washout in the presence of 1E16 (100 nM) only (C).