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Creative Biolabs

HOT NeuroMab™ Anti-CD32b Antibody(NRP-0422-P1803)

[CAT#: NRP-0422-P1803]

Functional antibody against Human CD32b

Host Species:
Mouse
Species Reactivity:
Human
Applications:
ELISA; WB; ADCC; Cyt; Block; In Vitro; In Vivo

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Product Overview

Immunogen

Recombinant soluble extracellular domain

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; WB; ADCC; Cyt; Block; In Vitro; In Vivo

Relevant Diseases

Systemic Lupus Erythematosus; Malaria
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

CD32b

Official Name

FCGR2B

Full Name

Fc fragment of IgG receptor IIb

Alternative Names

FCGR2B; CD32; CD32B; FCG2; FCGR2; IGFR2; Fc fragment of IgG receptor IIb; FcRII-c; FCGR2C

Gene ID

Uniprot ID

Product Pictures
ELISA

Figure 1 shows the binding of anti-CD32b antibodies to (Figure 1A) CD32bECDHis and (Figure 1B) CD32aECDHis measured by ELISA.

FuncS

Figure 10 shows the binding of anti-CD32b antibodies to human peripheral blood leukocytes.

WB

Figure 11 shows the binding of anti-CD32b antibody to CD32bECDHis in Western blot. Under reducing (lanes 1-6) and non-reducing conditions (9-12).

FCM

Figure 2 shows the binding of anti-CD32b antibodies to stably transfected IIA1.6 cells expressing full-length CD32b measured by flow cytometry.

ADCC

Figure 3 shows the ability of anti-CD32b antibody to induce Daudi cell lysis by ADCC compared to HuMab-KLH

FRET

Figure 4 shows CD32b TR-FRET based binding competition data. Maximum TR-FRET fluorescence was determined in samples without unlabeled mAb (which did not compete with labeled mAb). Background fluorescence was measured in samples without CD32bECDHis and CD32aECDHis. The inhibition of a-KLH-AlexaFluor 647 binding to CD32bECDHis and CD32aECDHis was represented by IC50 values (Table 6). The data for antibody 026 illustrate antibodies 020, 022, 024, 028, 053 and 063.

FuncS

Figure 5 shows the in vivo anti-tumor efficacy of anti-CD32b antibodies in a xenograft tumor model in SCID mice. Antibody was administered on day 0 (Figure 5A) or day 6 (Figure 5B) after tumor challenge; shown are mean counts per minute (cpm) values ± SEM for each treatment group.

FuncS

Figure 6 shows the in vivo anti-tumor efficacy of anti-CD32b antibodies in a xenograft tumor model in SCID mice. Antibody was administered on day 6 (Figure 6A and 6B) or 14 days after tumor challenge (Figure 6C); data shown are mean tumor burden ± SEM for each group.

FuncS

Figure 7 shows the ability of anti-CD32b antibodies to induce Daudi cell lysis by ADCC compared to HuMab-KLH. Data shown are mean ± SEM of triplicates.

FuncS

Figure 8 shows the binding of anti-CD32b antibodies to membrane-bound CD32b1 expressed on IIA1.6 cells. Data shown are mean MFI ± stdev of three independent experiments.

FuncS

Figure 9 shows the binding of anti-CD32b antibodies to mantle cell lymphoma cells.

Publications

Publications (0)

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