NeuroMab™ Mouse Anti-Human CRH Monoclonal Antibody (CBP1503)

Product Overview

Description

Mouse anti-human corticotropin releasing factor antibody recognizes human corticotropin releasing factor (CRF), which is a hormone mainly found in the hypothalamus and can regulate the release of pituitary gland corticosteroids.
The unique lysine residue (Lys-36) may be related to the antigenic determinant because the modification of lysine by CA completely blocks the binding. Human serum containing CRF binding protein also inhibits the binding of the antibody to CRF. Averages 10(e)-9 M with native and modified CRF.

Immunogen

Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1503

Applications

ELISA; IF; IHC-Fr; IHC-P

Application Notes

IHC-P (1/100-1/7000)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1330

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified Ig prepared by affinity chromatography on Protein G.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CRF

Official Name

CRH

Full Name

Corticotropin-releasing hormone

Alternative Names

CRH; CRF; CRH1; corticotropin releasing hormone

Gene ID

1392(Human); 12918(Mouse); 81648(Rat)

Uniprot ID

P06850(Human); Q8CIT0(Mouse); P01143(Rat)

References

1. Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.
2. DeBellis, A. et al. (2012) Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells.

Publications