NeuroMab™ Mouse Anti-Human CD81 Monoclonal Antibody (CBP2233)

Product Overview

Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

Immunogen

Human OCI-LY8 cell line

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2233

Applications

Application Notes

FCM (5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1658

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD81

Official Name

CD81

Full Name

Cluster of Differentiation 81

Alternative Names

CD81; CVID6; S5.7; TAPA1; TSPAN28; CD81 molecule

Gene ID

975 (Human); 12520 (Mouse)

Uniprot ID

P60033 (Human); P35762 (Mouse)

References

1. Menno C, et al. 2010. J. Clin. Invest. 4:1265.
2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127.
3. Wright M, et al. 1994. Immunol. Today 15:588.

Publications