NeuroMab™ Mouse Anti-GLI1 Monoclonal Antibody (CBP2868)

Product Overview

Description

GLI1 belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development.

Expression Host

Mammalian cells

Immunogen

GLI1 fusion protein Ag27832

Species Reactivity

Human; Rat; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2868

Applications

WB; ELISA

Application Notes

WB : 1:500-1:1000

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1897

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

GLI1

Official Name

GLI1

Full Name

GLI family zinc finger 1

Alternative Names

GLI; GLI family zinc finger 1; GLI1; Glioma associated oncogene; Oncogene GLI; Zinc finger protein GLI1

References

1. Ge, M., Zhang, L., Cao, L., Xie, C., Li, X., Li, Y.,... & Zhang, Q. (2019). Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway. International journal of food sciences and nutrition, 70(5), 570-578. 2. Sun, X., Song, J., Li, E., Geng, H., Li, Y., Yu, D., & Zhong, C. (2020). Cigarette smoke supports stemness and epithelial-mesenchymal transition in bladder cancer stem cells through SHH signaling. International Journal of Clinical and Experimental Pathology, 13(6), 1333. 3. Li, E., Zhang, T., Sun, X., Li, Y., Geng, H., Yu, D., & Zhong, C. (2019). Sonic hedgehog pathway mediates genistein inhibition of renal cancer stem cells. Oncology letters, 18(3), 3081-3091.

Publications