Human DAG1 Knockout HEK293T Cell Line

[CAT#: NCL2008ZP272]

Human DAG1 knockout cell line

Species:: Human

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This product NCL2008ZP272 is a human DAG1 gene knockout HEK293T cell line which achieved by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurogenesis

Species

Human

Properties

Size

1 x 10e6 cells/vial, 1 mL

Formulation

Constituents: 8.7% DMSO, 2% Cellulose, methyl ether

Growth Pattern

Adherent

STR Analysis

Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Biosafety Level

Tissue Source

Kidney

Cell Purity

>95%

Cell Viability

~90%

Antibiotic Resistance

Puromycin 1.00µg/ml

Mycoplasma Testing

Yes

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Shipped on Dry Ice. Store in liquid nitrogen.

Handling Advice

Upon arrival, the sample vial should be stored in liquid nitrogen vapor phase instead of -80°C. Storage at -80°C will cause loss of vitality.

1. Thaw the vial in a 37°C water bath for about 1-2 minutes.
2. Transfer the cell suspension to a 15 mL conical tube containing pre-warmed 5 mL complete medium DMEM + 10% FBS, and rotate 125 x g for about 5 minutes at room temperature.
3. Resuspend the cell pellet with 1 mL of pre-warmed complete medium DMEM + 10% FBS, and distribute it into a 25 cm2 culture flask, which contains 10 mL of pre-warmed complete medium DMEM + 10% FBS.
4. Incubate the culture with 5% CO2 in a 37°C incubator.
5. The recommended subculture ratio is 1:4-1:6. When cells grow at 80-90% confluence and divide, the cells should be passaged.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.

Target Details

Target

DAG1

Official Name

DAG1

Full Name

Dystroglycan 1

Alternative Names

Dystroglycan 1; Dystroglycan 1 (Dystrophin-Associated Glycoprotein 1); Dystrophin-Associated Glycoprotein-1; Dystrophin-Associated Glycoprotein 1; Alpha-Dystroglycan; Beta-Dystroglycan; Dystroglycan; 156DAG;

Gene ID

1605(Human); 13138(Mouse); 114489(Rat)

Uniprot ID

Q14118(Human); Q62165(Mouse); F1M8K0(Rat)

Publications

Publications (0)

gift-card

QUICK LINKS

Antibody Discovery Services

AD In Vitro Assay

Antibody-depends Neurological Disease Solutions

AD Biomarker Assay

For Research Use Only. Not For Clinical Use.

Product

Hot Products

Antibodies

Cells

Kits

Tracers

Gene Related Products

Biospecimens

Cell Assay Reagents