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Creative Biolabs
Product

Human DDR1 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP352]

Human DDR1 knockout cell lysate

Species:
Human
Cell Types:
HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human DDR1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 47 bp insertion in exon12 and Insertion of the selection cassette in exon12.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

DDR1

Official Name

DDR1

Full Name

Discoidin domain receptor family, member 1

Alternative Names

DDR1; CAK; CD167; DDR; EHGK2; MCK10; NEP; NTRK4; PTK3; PTK3A; RTK6; TRKE; discoidin domain receptor tyrosine kinase 1

Gene ID

780(Human)

Uniprot ID

Q08345(Human)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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