Human RH7777/EDG4 Stable Cell Line

[CAT#: NCL20120081CR]

EDG4 Lysophospholipid Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

Lysophosphatidic acid (LPA), a bioactive lipid produced by several cell types including postmitotic neurons and activated platelets, is thought to be involved in various biological processes, including brain development. Three cognate G-protein coupled receptors encoded by lpa(1)/lp(A1)/Edg-2/Gpcr26, lpa(2)/lp(A2)/Edg-4, and lpa(3)/lp(A3)/Edg-7 mediate the cellular effects of LPA. However, many LPA-induced responses, including phospholipase C activation, Ca2+ mobilization, adenylyl cyclase activation, proliferation, JNK activation, AKT activation, and stress fiber formation, were absent or severely reduced in embryonic fibroblasts derived from lpa(1)(-/-) lpa(2)(-/-) mice.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Signal Transduction

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

DMEM, 10% FBS, 100 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

EDG4/LAP2

Family

Lysophospholipid receptor

Strain

RH7777/EDG4 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

EDG4

Official Name

EDG4

Alternative Names

RH7777; EDG4

Gene ID

9170(Human)

Uniprot ID

Q9HBW0(Human)

References

1. Cheng, Z., Zhang, X., Huang, P., Huang, G., Zhu, J., Chen, F.,.. & Wang, X. (2020). Nup96 and HOS1 are mutually stabilized and gate CONSTANS protein level, conferring long-day photoperiodic flowering regulation in Arabidopsis. The Plant Cell, 32(2), 374-391.

Publications