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Creative Biolabs

NeuroMab™ Anti-EPHA2 BBB Shuttle Antibody(NRZP-1022-ZP3854)

[CAT#: NRZP-1022-ZP3854]

Host Species:
Mouse
Species Reactivity:
Human
Applications:
WB; In Vitro; In Vivo; Inhib

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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

WB; In Vitro; In Vivo; Inhib

Relevant Diseases

Glioblastoma Multiforme
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

EPHA2

Official Name

EPHA2

Full Name

EPH receptor A2 (ephrin type-A receptor 2)

Alternative Names

EPHA2; Epha2; AW545284; Eck; Myk2; Sek-2; Sek2; ARCC2; CTPA; CTPP1; CTRCT6; EPH receptor A2; ECK
Product Pictures
ELISA

Figure 1 Eph099B-208.261 can compete with EA2 for EphA2 binding in a competitive ELISA assay.

FuncS

Figure 2 Antibody to EphA2 inhibits the growth of malignant tumor cells in soft agar.

single dose of 5 µg/ml of Eph099B-208.261 (black bars), EA2 (white bars) purified EphA2 antibody, or negative control antibody 1A7 (gray bars) was incubated with malignant MDA-MB-231 tumor cells to achieve the indicated levels in soft agar. time at 37°C. Results are reported as colonies per high power field (HPF).

FuncS

Figure 3 EphA2 antibodies EA2, Eph099B-208.261 and Eph099B-233.152 inhibit tumor cell growth in vivo.

MDA-MB-231 breast cancer cells were implanted (A) orthotopically or (B) subcutaneously into athymic mice. (C) A549 lung cancer cells were implanted subcutaneously in athymic mice. After tumor growth to an average volume of 100 mm3, mice were administered 6 mg/kg of the indicated antibody or negative control (PBS or 1A7 antibody) intraperitoneally twice weekly for 3 weeks. Tumor growth was assessed and expressed as the ratio of tumor volume divided by initial tumor volume (100 mm3).

FuncS

Figure 4 EphA2 antibodies EA2, Eph099B-208.261 and Eph099B-233.152 inhibit tumor cell growth in vivo.

MDA-MB-231 breast cancer cells were implanted subcutaneously in athymic mice. After tumor growth to an average volume of 100mm3, mice were dosed intraperitoneally with 6 mg/kg of the indicated antibody or negative control twice a week for 3 weeks. Total tumor volume was determined after sacrifice. The negative control is black, EA2 is white, Eph099B-208.261 is dark gray, and Eph099B-233.152 is light gray.

SPR

Figure 5 Kinetic analysis of EphA2 monoclonal antibodies.

The BIACORE™ (surface plasmon resonance-based) assay was used to determine the kinetics of binding of EphA2 mAb to immobilized EphA2-Fc. Eph099B-208.261 is represented by a solid line, Eph099B-233.152 is represented by a dotted line, EA2 is represented by a dotted line, and the negative control is represented by squares.

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