NeuroMab™ Anti-GluA2/GluR2 glutamate receptor BBB Shuttle Antibody, Clone L21/32

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-GRIA2 BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 834-883 (EFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGY NVYGIESVKI, cytoplasmic C-terminus) of rat GluA2/GluR2 (also known as Glutamate receptor 2, AMPA-selective glutamate receptor 2, Glutamate receptor ionotropic AMPA 2, GluR-B, GluR-K2 and Gria2, accession number P19491)
Mouse: 98% identity (49/50 amino acids identical)
Human: 98% identity (49/50 amino acids identical)
100% identity between Flip and Flop isoforms
>70% identity with GluA3/GluR3 and less identity with GluA1/GluR1 and GluA4/GluR4

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Rat; Mouse; Human

Clonality

Monoclonal

Isotype

IgG1

Clone Number

L21/32

Applications

WB; IHC; ICC

Application Notes

Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results)

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

GRIA2

Official Name

GRIA2

Full Name

GluA2/GluR2 glutamate receptor

Alternative Names

GRIA2; GLUR2; GLURB; GluA2; GluR-K2; HBGR2; glutamate ionotropic receptor AMPA type subunit 2; gluR-B; gluR-2

Uniprot ID

P19491

Product Pictures

Fig. 1 Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with L21/32 (left) or N52A/42 (right) TC supe.

Fig. 2 Immunoblot of membranes from adult rat brain (RBM) and adult GluA2/GluR2 knockout (KO) and wild-type (WT) mouse hippocampi (MHM).

Fig. 3 Adult rat brain membrane immunoblot.

IHC

Fig. 4 Adult rat hippocampus immunohistochemistry.

IEM

Fig. 5 Electron micrograph of L21/32 hippocampal labelling using a postembedding immunogold method. Immunoparticles (arrows) were observed in the postsynaptic densities of dendritic spines and dendritic shafts (Den) establishing asymmetrical synapses with axon terminals (b).

Publications