NeuroMab™ Anti-Notch 1 Antibody(NRP-0422-P1182)

Product Overview

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Applications

FC; In Vitro; Inhib

Relevant Diseases

Alzheimer's Disease; Epilepsy; Pain

Product Properties

Preservatives

BSA Free

Concentration

1mg/mL

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Notch1

Official Name

NOTCH1

Full Name

NOTCH1

Alternative Names

Notch 1; Translocation-Associated Notch Protein TAN-1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1; Translocation-Associated (Drosophila); Notch Homolog 1; Translocation-Associated;

Gene ID

4851 (Human); 18128 (Mouse); 25496 (Rat)

Uniprot ID

P46531 (Human); Q01705 Mouse (Mouse); Q07008 Rat (Rat)

Product Pictures

FuncS

Figure 1: Binding of anti-NOTCH1 monoclonal antibodies 13M57 and 31M80 to cell surface expressed NOTCH1.

FACS analysis of mock-transfected cells or cells expressing the full-length NOTCH1 receptor incubated with the anti-NOTCH1 receptor antibody 31M80 demonstrated that 31M80 specifically recognizes the NOTCH1 receptor.

FuncS

Figure 2 Epitope mapping of anti-NOTCH1 mAbs binding to the non-ligand binding domain.

FuncS

Figure 3: Binding of anti-NOTCH1 monoclonal antibodies 13M57 and 31M80 to cell surface expressed NOTCH1.

Anti-NOTCH1 antibodies 13M57 and 31M80 showed increased binding to PE13 breast tumor cells compared to an isotype control antibody (bottom) and this binding corresponded to cells that express high levels of ESA and CD44 (top: CD44 x-axis and NOTCH1 y-axis).

FuncS

Figure 4: Binding of anti-NOTCH1 monoclonal antibodies 13M57 and 31M80 to cell surface expressed NOTCH1.

Anti-NOTCH1 antibody 31M80 showed increased binding to dissociated colon tumor cells from two different patients compared to an isotype antibody control.

Publications