NeuroMab™ Anti-Notch 1 Antibody(NRP-0422-P1831)

Product Overview

Description

The antibody specifically binds to Notch1 NRR. The anti-Notch1 NRR antibody inhibits tumor growth in mouse model.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Humanized

Applications

ELISA; WB; IHC; Inhib; In Vitro

Relevant Diseases

Alzheimer's Disease; Epilepsy; Pain

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

Notch1

Official Name

NOTCH1

Full Name

NOTCH1

Alternative Names

Notch 1; Translocation-Associated Notch Protein TAN-1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1; Translocation-Associated (Drosophila); Notch Homolog 1; Translocation-Associated;

Gene ID

4851 (Human); 18128 (Mouse); 25496 (Rat)

Uniprot ID

P46531 (Human); Q01705 Mouse (Mouse); Q07008 Rat (Rat)

Product Pictures

Neut

Figure 1 shows the neutralizing activity of humanized 438 VH1.1/VL1.8 and rat 438 antibodies against Notch1-dependent signaling in human Notch1 reporter cells.

Neut

Figure 2 shows the neutralizing activity of humanized 438 VH1.1/VL1.8 and rat 438 antibodies on Notch1-dependent signaling in mouse Notch1 reporter cells.

Neut

Figure 3 shows the neutralizing activity of humanized 351 mutant and rat 351-mIgG1 in a human Notch1-dependent signaling reporter assay.

Neut

Figure 4 shows the neutralizing activity of humanized 351 mutants and rat 351-mIgG1 in a mouse Notch1-dependent signaling reporter assay.

Figure 5 Treatment of HBP-ALL cells with increasing concentrations of rat 438-mIgG1 and humanized 438VH1.1/VL1.8 significantly inhibited constitutive Notch1 activation.

IHC

Figure 6 shows the histochemical identification of secretory goblet cells using Alcian blue staining on ileal sections of mouse intestine obtained from a Calu-6 efficacy study.

IHC

Figure 7 shows representative Figures of intestinal crypts stained with Ki67, where Ki67 staining was reduced in animals treated with rat 351-mIgG1 at the bottom of the crypts

Publications