NeuroMab™ Anti-TLR4 BBB Shuttle Antibody(NRZP-1022-ZP2667)

Fig.1 is a graph depicting the binding by monoclonal phages expressing scfv to the human TLR4/MD2 complex.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.2 is a graph depicting the binding by purified antibodies to the human TLR4/MD2 complex.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.3 is a graph depicting the binding by purified antibodies, referred to herein as “1E11, 1E11 N103D”, to the cynomolgus monkey TLR4.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.4 is a graph depicting the binding by purified antibodies, referred to herein “1E11, 1E11 N103D”, to the human TLR4/MD2 complex.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.5 is a graph depicting the inhibition of LPS-induced downstream signaling cascade of TLR4, NF-κB, by purified antibodies, referred to herein “1E11, 15C1, 1C12, 1G12”.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.6 is a graph depicting the binding potency of purified antibodies with CDRH1 mutations, referred to herein “15C1, 1E11.C2E1, 1E11.C2E3, 1E11.C2E4, 1E11.C2E5”, to the human TLR4/MD2 complex.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.

Fig.7 is a series of graphs depicting the inhibition of IL-6 production induced by TLR4 activation in cynomolgus monkey whole blood assay by purified antibodies, referred to herein “15C1, 1E11C2”.

The cells were incubated with either mu18H10, HTA 125 (a commercially available anti-human TLR4 non-blocking MAb) or an antibody control at the indicated concentrations and subsequently incubated with LPS (15 ng/ml). IL-8 levels were assessed 16 hours post LPS treatment.