NeuroMab™ Anti-TREM2 Antibody(NRP-0422-P1403)

Figure 3 shows the induction of a mouse TREM2-dependent luciferase reporter gene in a cell-based assay.

Cells were either untreated (NT) or treated with soluble full-length anti-TREM2 antibodies 1H7, 2F6, 2H8, 3A7, 3B10, 7E5, 7F8, 8F8, and 11H5. Antibody mlgG1 is an isotype negative control. Cells treated with PMA/ionomycin represent a positive control. Results are represented as folded backgrounds (indicated by dashed lines).

Figure 6 shows the percent survival (%) of human monocyte-derived dendritic cells incubated with soluble TREM2 antibodies 9F5 or 10A9.

In contrast to antibody 10A9, the survival of dendritic cells was not significantly reduced after incubation with antibody 9F5. "mlgG1" refers to the mouse isotype control antibody, and "medium" refers to the media-only control.

Figure 1 shows FACS histograms demonstrating that the human-specific TREM2 antibodies 9F5 and 10A9 bind to human TREM2 expressed on macrophages of humanized TEM2 BAC transgenic mice (huTREM2 Tg), but not wild-type mice (WT ) macrophage binding.

Figure 2 shows the full length anti-TREM2 antibodies 9F5, 9G1, 9G3, 10A9, 10C1, 11A8, 12D9, 12E2, 12F9, 12G6, 2C7, 2F5, 3C1 and 4D7 in solution.

Antibody mlgG1 is an isotype negative control. Cells treated with PMA/ionomycin represent a positive control. Results are represented as folded backgrounds (indicated by dashed lines).

Figure 4 shows the induction of the human TREM2-dependent luciferase reporter gene in a cell-based assay.

Cells were untreated (NT) or treated with plate-bound full-length anti-TREM2 antibodies 9F5, 9G1, 9G3, 10A9, 10C1, 11A8, 12D9, 12E2, 12F9, 12G6, 2C7, 2F5, 3C1, and 4D7. Results are expressed as background multiples. Antibody msIgG1 is an isotype negative control. Cells treated with PMA/ionomycin (P+I) represent a positive control.

Figure 5 shows FACS histograms demonstrating binding of TREM2 antibodies 10A9, 10C1 and 8F8 to human cell lines (293) expressing recombinant human TREM2-DAP12 fusion protein.

Shaded histograms represent TREM2-negative cell populations. Histograms outlined in black represent the TREM2-positive cell population.