Creative Biolabs

iNeu Microglia AD TREM2 HO Kit, Disease Model

[CAT#: NCL-7P012]

Microglia Alzheimer's Disease differentiated from Human iPS cells, frozen

Cell Assay
Cell Types:

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Microglia are immune cells that reside in the central nervous system (CNS) and are responsible for basic physiological and pathological processes. Microglia support neuronal homeostasis and promote the formation of neuronal networks through synaptic pruning. Surveillance Microglia can activate, adapt and respond to specific stimuli accordingly.

Microglial dysfunction is considered a key factor in neurodegenerative diseases, including Alzheimer's disease (AD). The limited availability and inconsistency of primary Human microglia limit research and treatment progress.

A rare variant of TREM2 (trigger receptor 2 expressed on bone marrow cells), an immune receptor expressed on microglia, is known to trigger bacterial phagocytosis and the release of reactive oxygen species, and is associated with AD patients The increased risk is related. Most TREM2 mutations found in AD risk variants are heterozygous mutations that affect TREM2 ligand binding or shedding of extracellular domains. The loss-of-function mutations of TREM2 and adaptor protein TYROBP are also related to the development of Nasu-Hakola disease, which is an inflammatory degenerative disease of the brain and bones, leading to premature dementia and death.

Engineering TREM2 model
In order to be able to study the functional consequences of TREM2 variants, the frameshift of TREM2 exon 2 is at amino acids near either allele (heterozygous; C60MfsTer45; iNeu Microglia TREM2 HZ) or two alleles (homozygous; P59AfsTer16 and P59AfsTer46) HO00) is designed in iPSCs. These iPSCs come from an apparently healthy and normal donor background, 01279.
Cell Types
Cell Assay
1 Vial: ≥ 1 x 10^6 cells/vial
Cryopreserved iNeu Microglia TREM2 (HO), 01279
50ml iNeu Glial Base Medium
0.5ml iNeu Microglia Supplement A (100x)
0.5ml iNeu Microglia Supplement B (100x)
2 x 1ml iNeu Neural Supplement C (25X)
iNeu Microglia Quick Guide
Additional reagents required; see Quick Guide
Tissue Source
Mycoplasma Testing
The cell line has been screened using the Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination.
Dry ice.
Liquid Nitrogen
Research Use Only
For research use only
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Official Name
Full Name
; A2 Thromboxane Receptor;
Alternative Names
A2A receptor; A2a; ADORA2; RDC8; A2ar; Adenosine A2a-receptor; A2AAR; AA2AR; Rs
Donor Health
Alzheimer's Disease
Additional Donor Information
Zygosity: Homozygous
Genotype: TREM2 (KO)

For Research Use Only. Not For Clinical Use.
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