NeuroMab™ Mouse Anti-LAMA2 Monoclonal Antibody (CBP1513)

Description

The mouse anti-human laminin alpha 2 antibody recognizes laminin subunit α-2 (also known as laminin M chain, laminin-12 subunit α, laminin-2 subunit α, laminin-4 subunit α or merosin heavy chain) ~ 80 kDa fragment. Laminin is a trimeric basic glycoprotein composed of three different multi-domain chains (α, β, and γ), each of which is encoded by a different gene. The alpha-2 chain is a subunit of Laminin-2 and Laminin-4, and is widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminin is thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components. Mutations in the LAMA2 gene can lead to the development of myosin-deficient congenital muscular dystrophy 1A (MDC1A), which is characterized by hypotonicity, proximal weakness, insufficient reflexes, and difficulty walking.

Immunogen

Purified Human Merosin.

Species Reactivity

Human; Rabbit; Monkey

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1513

Applications

ELISA; IF; IHC-Fr; IHC-P; IP; WB

Application Notes

ELISA (1/25000-1/100000); IHC-Fr (1/2000-1/10000); IHC-P (1/50)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1331

Form

Ascitic Fluid - raw

Formulation

Ascitic Fluid-raw
0.09% Sodium Azide

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

Laminin Alpha 2

Official Name

LAMA2

Full Name

Laminin subunit alpha-2

Alternative Names

LAMA2; LAMM; Laminin; alpha 2; laminin subunit alpha 2; MDC1A

Gene ID

16773(Mouse)

Uniprot ID

Q60675(Mouse)

1. Sewry, C.A. et al. (1995) Expression of laminin subunits in human fetal skeletal muscle. 2. Engvall, E. et al. (1990) Distribution and isolation of four laminin variants; tissue restricted distribution of heterotrimers assembled from five different subunits. 3. Tan, E. et al. (1997) Late onset muscular dystrophy with cerebral white matter changes due to partial merosin deficiency. 4. Awamura, Y. et al. (2008) Long-term follow-up of laminin alpha2 (merosin)-deficient muscular dystrophy in a cat. 5. Eriksson, A. et al. (2005) Skeletal muscle morphology in power-lifters with and without anabolic steroids. 6. Guo, L.T. et al. (2003) Laminin alpha2 deficiency and muscular dystrophy; genotype-phenotype correlation in mutant mice. 7. Kjellgren, D. et al. (2004) Laminin isoforms in human extraocular muscles. 8. Li, J. et al. (2006) Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions. 9. Marinkovich, M.P. et al. (1992) The dermal-epidermal junction of human skin contains a novel laminin variant. 10. Nakano, J. et al. (2005) Laminin-induced autoimmune myositis in rats. 11. O'Brien DP et al. (2001) Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats. 12. Tian, M. et al. (1997) Laminin-alpha2 chain-like antigens in CNS dendritic spines. 13. Tran, T. et al. (2006) Endogenous laminin is required for human airway smooth muscle cell maturation. 14. Vainionpää, N. et al. (2007) Basement membrane protein distribution in LYVE-1-immunoreactive lymphatic vessels of normal tissues and ovarian carcinomas. 15. Vuoristo, S. et al. (2009) Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support. 16. Alhamidi, M. et al. (2017) Limb Girdle Muscular Dystrophy type 2I: The clinical variability seen in patients homozygous for the common FKRP (c.826C>A) mutation does not correlate with histopathological alterations, levels of glycosylated α-dystroglycan or laminin α2 in vastus lateralis.