NeuroMab™ Rat Anti-Human MINA Monoclonal Antibody (CBP2206)

Product Overview

Description

MINA, also known as Mineral dust-induced gene (MDIG), is a member of the JmjC family of proteins and can act as a histone lysine demethylase and a ribosomal histidine hydroxylase. MINA is implicated in the demethylation of K9 trimethylated histone H3 (H3K9me3), regulating cell proliferation and survival. MINA has been suggested to be a novel oncogene associated with a variety of cancers. Elevated expression of MINA in human tumors has been linked to malignancy and poor prognosis. MINA has been identified as a transcriptional co-repressor of IL-4 gene and participates in the regulation of Th2 differentiation and Th2 bias. MINA appears to be a regulator of the Treg/Th17 balance by promoting differentiation of Th-17 cells and inhibiting FOXP3 expression.

Immunogen

Partial recombinant protein (300-465 aa) expressed in E. coli.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP2206

Applications

WB; ICC

Application Notes

WB (0.5-2.5 µg/mL); ICC (1.0-5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1635

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

MINA

Official Name

KRT18

Full Name

Keratin 18

Alternative Names

KRT18; CK-18; CYK18; K18; keratin 18

Gene ID

3875 (Human); 16668 (Mouse)

Uniprot ID

P05783 (Human); P05783 (Mouse)

References

1. Thakur C, et al. 2015. Genes Cancer 6:288.
2. Pillai MR, et al. 2014. Curr. Opin. Immunol. 31:38.
3. Lian S, et al. 2013. PLoS One 8:e80638.
4. Hemmers S, et al. 2009. Nat. Immunol. 10:806.
5. Lu Y, et al. 2009. Cell Cycle. 8:2101.
6. Tsuneoka M, et al. 2004. Clin. Cancer Res. 10:7347.

Publications